M. Haqqul Syifa' Masyhur Hakim, . (2026) PENGARUH EKSTRAK ETANOL DAUN UNGU GRAPTOPHYLLUM PICTUM TERHADAP STROKE PADA LARVA ZEBRAFISH YANG DIINDUKSI PONATINIB. Tesis thesis, Universitas Pembangunan Nasional Veteran Jakarta.
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Abstract
Background: Stroke remains a leading cause of death worldwide. The limitations of animal models in representing human pathology pose a significant challenge for translational stroke medicine. Ponatinib-induced zebrafish (Danio rerio) offer a potential alternative model for ischemic stroke. Purple leaves (Graptophyllum pictum) show promise as a stroke therapy due to bioactive compounds with anti-platelet aggregation properties. Methods: An acute toxicity test of the Graptophyllum pictum ethanol extract was conducted on zebrafish embryos at 6 hours post-fertilization (hpf) following OECD Guideline No. 236 to determine LC50 and LC10 values. Zebrafish larvae at 2 days post-fertilization (dpf) were divided into eight groups. The larvae were induced with 1 µg/mL ponatinib and treated with Graptophyllum pictum ethanol extract at doses of ¼ LC10, ½ LC10, and LC10. Larvae were also treated with 12.5 µg/mL edaravone as a positive control and 0.1% DMSO as a vehicle control. Evaluations were performed regarding larval motility, cerebral edema, cell apoptosis, and thrombus area. Results: The toxicity test showed an LC50 value of 77.37 μg/mL (slightly toxic category) and an LC10 value of 38.107 μg/mL for the Graptophyllum pictum ethanol extract. Induction with 1 µg/mL ponatinib successfully triggered ischemic stroke, characterized by decreased motility, increased brain edema, thrombus formation, and increased brain cell apoptosis. Treatment with Graptophyllum pictum ethanol extract at a dose of 38.107 μg/mL significantly restored larval motility, reduced brain edema, and decreased cellular apoptosis, showing comparable effectiveness to 12.5 µg/mL edaravone (p<0,05). The dose of 9.52 μg/mL was identified as the lowest safe and effective dose to significantly reduce the brain thrombus area. Conclusion: The ethanol extract of Graptophyllum pictum has the potential to treat ponatinib-induced ischemic stroke in zebrafish larvae by reducing thrombus area, alleviating cerebral edema, preventing cell apoptosis, and restoring larval motility.
| Item Type: | Thesis (Tesis) |
|---|---|
| Additional Information: | [No.Panggil: 2410223002] [Pembimbing 1: Feda Anisah Makkiyah] [Pembimbing 2: Ria Maria Theresa] [Penguji 1: Tiwuk Susantiningsih] [Penguji 2: Muttia Amalia] |
| Uncontrolled Keywords: | Graptophyllum pictum, Purple Leaves, Zebrafish, Ischemic Stroke, Ponatinib. |
| Subjects: | R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Fakultas Kedokteran > Sains Biomedis (S2) |
| Depositing User: | M. HAQQUL SYIFA' MASYHUR HAKIM |
| Date Deposited: | 01 Sep 2026 07:19 |
| Last Modified: | 01 Sep 2026 07:19 |
| URI: | http://repository.upnvj.ac.id/id/eprint/53235 |
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