ISOLASI BAKTERI RIZOSFER TANAH KELOR (MORINGA OLEIFERA) SEBAGAI SUMBER ANTIOKSIDAN POTENSIAL DALAM PENDEKATAN PREVENTIF DIABETES MELITUS TIPE 2

Sayidah Alfiah Ziaur Rahmah, . (2026) ISOLASI BAKTERI RIZOSFER TANAH KELOR (MORINGA OLEIFERA) SEBAGAI SUMBER ANTIOKSIDAN POTENSIAL DALAM PENDEKATAN PREVENTIF DIABETES MELITUS TIPE 2. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

Rhizospheric bacteria are known to produce bioactive metabolites, including antioxidant compounds. Oxidative stress plays an important role in the pathogenesis of Type 2 Diabetes Mellitus (T2DM), making the exploration of natural antioxidant sources relevant within a preventive approach. This study explores the potential of rhizospheric bacteria isolated from Moringa oleifera soil as a source of antioxidant compounds related to oxidative stress in T2DM. Objective: To assess the potential of rhizospheric bacteria from Moringa oleifera soil as a source of antioxidant activity within a preventive approach to Type 2 Diabetes Mellitus through in vitro evaluation. Methods: This study employed a descriptive experimental design. Rhizospheric bacteria were isolated using culture methods on Nutrient Agar to obtain pure isolates. Preliminary phenotypic characterization was conducted based on colony and cell morphology, Gram staining, and catalase testing. A selected isolate was fermented in Nutrient Broth, and metabolites were extracted using Ultrasound-Assisted Extraction (UAE). Antioxidant activity was evaluated in vitro using the DPPH assay, with vitamin C as a positive control. Data were analyzed descriptively and presented as mean percentage inhibition. Results: Three rhizospheric bacterial isolates with distinct phenotypic characteristics were obtained, including two Gram-positive isolates (cocci and bacilli) and one Gram-negative isolate (coccobacillus). All isolates showed positive catalase activity. The DPPH assay demonstrated antioxidant activity of the fermented metabolite extract at all tested concentrations (20%, 40%, 60%, and 80%), with the highest inhibition observed at 40% concentration (45.4%). The concentration–activity relationship was non-linear, and maximum inhibition did not reach 50%. Conclusion: Rhizospheric bacteria isolated from Moringa oleifera soil exhibit preliminary in vitro antioxidant activity, serving as an initial indicator of antioxidant potential related to oxidative stress within a preventive approach to Type 2 Diabetes Mellitus. Further studies are required to confirm and develop this potential.

Item Type: Thesis (Skripsi)
Additional Information: [No.Panggil: 2210211103 [Pembimbing 1 : Desi Purwaningsih] [Pembimbing 2 : Diana Agustini Purwaningastuti] [Penguji : Tiwuk Susatiningsih]
Uncontrolled Keywords: Antioxidant; DPPH; Moringa oleifera; rhizosphere bacteria; T2DM; Ultrasound-Assisted Extraction (UAE)
Subjects: R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: SAYIDAH ALFIAH ZIAUR RAHMAH
Date Deposited: 06 May 2026 07:08
Last Modified: 06 May 2026 07:08
URI: http://repository.upnvj.ac.id/id/eprint/41698

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