Octaria Bulan Kusuma Putri, . (2024) OPTIMASI ELUEN DAN VALIDASI METODE ANALISIS PADA UJI KADAR KUERSETIN TERHADAP EKSTRAK KERING DAUN KELOR (Moringa oleifera L.). Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.
![[img]](http://repository.upnvj.ac.id/style/images/fileicons/text.png) |
Text
ABSTRAK.pdf Download (225kB) |
|
Text
AWAL.pdf Download (570kB) |
|
Text
BAB 1.pdf Restricted to Repository UPNVJ Only Download (207kB) |
|
Text
BAB 2.pdf Restricted to Repository UPNVJ Only Download (634kB) |
|
Text
BAB 3.pdf Restricted to Repository UPNVJ Only Download (405kB) |
|
Text
BAB 4.pdf Restricted to Repository UPNVJ Only Download (407kB) |
|
Text
BAB 5.pdf Download (185kB) |
|
Text
DAFTAR PUSTAKA.pdf Download (204kB) |
|
Text
RIWAYAT HIDUP.pdf Restricted to Repository UPNVJ Only Download (149kB) |
|
Text
LAMPIRAN.pdf Restricted to Repository UPNVJ Only Download (1MB) |
|
Text
HASIL PLAGIARISME..pdf Restricted to Repository staff only Download (2MB) |
|
Text
ARTIKEL KI.pdf Restricted to Repository staff only Download (449kB) |
Abstract
The Moringa plant (Moringa oleifera L.) has many nutritional benefits and pharmacological activities, especially due to the quercetin content found in its leaves. This research aimed to determine the optimal eluent ratio from a combination of methanol and aquabides for testing quercetin levels in dry Moringa leaf extract samples. Additionally, the analytical method was validated to ensure it met the requirements for linearity, precision, accuracy, specificity, and range. The research method involved varying the eluent composition of methanol at ratios of 60:40, 65:35, and 70:30 using a High Performances Liquid Chromatography (HPLC) instrument. The results showed that the optimal eluent ratio was methanol:aquabides (60:40), based on parameters such as retention time (tR), tailing factor (As), area (uAu), and capacity factor (k). The analytical method validation results met the requirements set out in ICH Q2 (R1), with a retention time (tR) value of 2.25 minutes, follow-up factor (As) of 0.83, peak area of 28,764 ± 159.7, and capacity factor (k) of 7.81 ± 0.11. Based on this research, it can be concluded that the optimal eluent ratio is 60:40, the method used has met the validation requirements, and the dry extract of Moringa leaves obtained through the Heat-assisted Extraction process at a temperature of 60°C with 96% ethanol solvent contains quercetin at (22.68 ± 0.30) %.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Additional Information: | [No. Panggil: 2010212019] [Pembimbing utama: Via Rifkia] [Pembimbing pendamping: Dhigna Luthfiyani CP] [Penguji utama: Rika Revina] |
| Uncontrolled Keywords: | Eluent; HPLC; Moringa leaves; Quercetin; Validation method analysis. |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Fakultas Kedokteran > Program Studi Farmasi (S1) |
| Depositing User: | OCTARIA BULAN KUSUMA PUTRI |
| Date Deposited: | 09 Sep 2024 02:47 |
| Last Modified: | 09 Sep 2024 02:47 |
| URI: | http://repository.upnvj.ac.id/id/eprint/32629 |
Actions (login required)
 |
View Item |