ANALISIS PENGARUH VOLUME DAN TEKNIK TRANSCARDIAL PERFUSION FIXATION TERHADAP KUALITAS HISTOLOGIS OTAK TIKUS SPRAGUE-DAWLEY PADA MODEL OTAK PENELITIAN NEUROLOGIS

Dhyani Paramita Wahyudi, . (2026) ANALISIS PENGARUH VOLUME DAN TEKNIK TRANSCARDIAL PERFUSION FIXATION TERHADAP KUALITAS HISTOLOGIS OTAK TIKUS SPRAGUE-DAWLEY PADA MODEL OTAK PENELITIAN NEUROLOGIS. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

[img] Text
ABSTRAK.pdf
Download (14kB)
[img] Text
AWAL.pdf
Download (766kB)
[img] Text
BAB I.pdf
Restricted to Repository UPNVJ Only
Download (179kB)
[img] Text
BAB II.pdf
Restricted to Repository UPNVJ Only
Download (427kB)
[img] Text
BAB III.pdf
Restricted to Repository UPNVJ Only
Download (392kB)
[img] Text
BAB IV.pdf
Restricted to Repository UPNVJ Only
Download (1MB)
[img] Text
BAB V.pdf
Download (134kB)
[img] Text
DAFTAR PUSTAKA.pdf
Download (263kB)
[img] Text
RIWAYAT HIDUP.pdf
Restricted to Repository UPNVJ Only
Download (152kB)
[img] Text
LAMPIRAN.pdf
Restricted to Repository UPNVJ Only
Download (1MB)
[img] Text
HASIL PLAGIARISME.pdf
Restricted to Repository staff only
Download (2MB)
[img] Text
ARTIKEL KI.pdf
Restricted to Repository staff only
Download (589kB)

Abstract

Objective: Transcardial perfusion is widely used to preserve rodent brain tissue. However, varying parameters in protocols such as fixative volume and delivery technique remain unexplored, particularly its histological quality. This study aims to evaluate the effects of volume and delivery technique to the histological quality of rat brain tissue. Methods: Sixteen male Sprague-Dawley rats were allocated into four groups consisting of manual-200mL, manual-650mL, pump-200mL, and pump-650mL. Brains were processed with Hematoxylin-Eosin staining and photographed. Quantitative assessment of nuclear & cytoplasmic staining intensity was performed using Fiji. Histological artifacts (e.g., blood pooling) were scored. Group differences were analyzed using IBM SPSS Statistics. Results: Nuclear staining intensity produced the best value in the manual-200mL group (103.31 ± 19.29) and worst in the manual-650mL group (156.10 ± 14.94). Welch ANOVA confirmed a significant group effect (p < 0.001) between the manual-200mL with the manual-650mL group and the pump-200mL with the manual-650mL group. Cytoplasmic staining intensity showed the same trend. Tukey HSD indicates significance (p < 0.001) between the same groups as well as manual-200mL and pump-650mL. Artifact counts were descriptively higher in the pump groups, but showed no significant association (p = 0.362). Conclusion: Perfusion volume and technique influenced staining intensity, with manual 200 mL producing the best overall histological quality.

Item Type: Thesis (Skripsi)
Additional Information: [No. Panggil: 2210211087] [Pembimbing 1: Feda Annisa Makkiyah] [Pembimbing 2: Muttia Amalia [Penguji: Basuki Supartono]
Uncontrolled Keywords: transcardial perfusion, hematoxylin & eosin, brain model, fixation.
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: DHYANI PARAMITA WAHYUDI
Date Deposited: 21 Apr 2026 07:23
Last Modified: 21 Apr 2026 07:23
URI: http://repository.upnvj.ac.id/id/eprint/41937

Actions (login required)

View Item View Item