Prisilia Qurratu Aini, . (2026) EFEKTIVITAS SEL T SITOTOKSIK DALAM INDUKSI KEMATIAN GALUR SEL KANKER PAYUDARA MCF-7 SECARA IN VITRO. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.
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Abstract
Objective: Breast cancer remains the most prevalent malignancy among women in Indonesia, with 66,271 new cases annually and 70.9% diagnosed at advanced stages. The limitations of conventional therapies have driven the exploration of T cell-based immunotherapy as a promising alternative modality. This study aimed to evaluate the effectiveness of endogen ous cytotoxic T cells from breast cancer patients in inducing MCF-7 cell line death in vitro without genetic or pharmacological modification. Methods: This quantitative analytical experimental study was conducted in vitro. Cytotoxic T cells were isolated from breast cancer patient PBMCs, activated with anti-CD3, and ko-kultur d with MCF-7 cells for 72 hours at Effector:Target ratios of 10:1, 20:1, and 50:1. Evaluation was performed using inverted microscopy (morphology), Annexin V-PI flow cytometry (viability/apoptosis), and qRT-PCR (BCL-2 and p53 expression). Statistical analysis employed ANOVA and Tukey's test (α=0.05). Results: T cell characterization showed CD3+/CD8+ population increase from 5.26% to >94% and NKG2D+ >98% post-activation. At 10:1 and 20:1 ratios, viability decreased minimally (86-87%) with apoptosis dominance (apoptosis:necrosis ratio 2.5:1). At 50:1 ratio, viability decreased dramatically (38.24%) but was dominated by necrosis (50%). BCL-2 expression decreased significantly across all treatments (p<0.0001): 56% (10:1), 51% (20:1), 20% (50:1). p53 expression showed massive ownregulation of 96-99% across all ratios (p<0.0001), indicating a p53-independent cytotoxic mechanism. Conclusion: Endogen ous cytotoxic T cells effectively induced MCF-7 cell death. Ratios of 10:1 and 20:1 produced programmed apoptosis with limited cytotoxicity, while 50:1 ratio achieved high killing efficacy but was dominated by non-specific necrosis. Exploration of intermediate ratios (30:1-40:1) is recommended for optimization.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Additional Information: | [No Panggil : 2210211039] [Pembimbing 1 : Karina] [Pembimbing 2 : Niniek Hardini] [Penguji : Maulidya Ayudika Dandanah] |
| Uncontrolled Keywords: | cytotoxic T cells, MCF-7, breast cancer, apoptosis, immunotherapy, in vitro |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RA Public aspects of medicine R Medicine > RD Surgery |
| Divisions: | Fakultas Kedokteran > Program Studi Kedokteran (S1) |
| Depositing User: | PRISILIA QURRATU AINI |
| Date Deposited: | 08 May 2026 03:07 |
| Last Modified: | 08 May 2026 06:11 |
| URI: | http://repository.upnvj.ac.id/id/eprint/41857 |
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