PENGARUH PERBEDAAN FREKUENSI Ultrasound Assisted Extraction (UAE) TERHADAP EFEKTIVITAS EKSTRAK DAUN UNGU (Graptophyllum pictum (L.) Griff) DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Salmonella typhi

Annisa Fitria Nur, . (2024) PENGARUH PERBEDAAN FREKUENSI Ultrasound Assisted Extraction (UAE) TERHADAP EFEKTIVITAS EKSTRAK DAUN UNGU (Graptophyllum pictum (L.) Griff) DALAM MENGHAMBAT PERTUMBUHAN BAKTERI Salmonella typhi. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

Salmonella typhi is the bacteria that causes typhoid fever which has a fairly high incidence in the world. The emergence of multidrug-resistant S. typhi (MDRST) poses a new threat to the successful management of typhoid fever. Purple leaves (Graptophyllum pictum (Linn) Griff) contain the bioactive compounds such as tannin, flavonoids, alkaloids and saponins, which are known to have antibacterial activity. The aim of this research was to determine the effect of different ultrasonic frequencies on the effectiveness of purple leaf extract as an antibacterial against S. typhi. The extraction was carried out using the ultrasound assisted extraction (UAE) method with varying frequencies of 30 kHz, 40 kHz, and 50 kHz with 70% ethanol solvent, temperature 45°C, and extraction time of 20 minutes. This research method is true experimental and was carried out in vitro. The results showed that purple leaves extracted using the ultrasonic method at frequencies of 30 kHz, 40 kHz and 50 kHz had the ability to inhibit the growth of S. typhi bacteria with an average diameter of the inhibition zone of 3.67 mm; 8.39 mm; and 5.52 mm. Data analysis using the Kruskal Wallis test showed that there was at least one pair of groups that had a significant difference in the average zone of inhibition (P<0.05). Mann Whitney Post-Hoc test showed that there were significant differences in each pair of treatment groups (P<0.05). It can be concluded that different ultrasonic frequencies influence the effectiveness of purple leaf extract in inhibiting the growth of S. typhi and the most optimal frequency is 40 kHz, which has the best cavitation effect in breaking down purple leaves cell walls so that maximum target compounds can be obtained. Keywords: Antibacterial, purple leaves, Salmonella typhi, ultrasound assisted extraction.

Item Type: Thesis (Skripsi)
Additional Information: [No. Panggil : 2010211086} [Pembimbing 1 : Meiskha Bahar] [Pembimbing 2 : Yanti Harjono Hadiwiardjo] [Penguji : Yuni Setyaningsih]
Uncontrolled Keywords: Antibacterial, purple leaves, salmonella typhi, ultrasound assisted extraction.
Subjects: Q Science > QR Microbiology
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: Annisa Fitria Nur
Date Deposited: 20 Mar 2024 04:13
Last Modified: 20 Mar 2024 04:13
URI: http://repository.upnvj.ac.id/id/eprint/28542

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