R. Regina Zaafira Desnita Purnawan, . (2024) ANALISIS GEN BAX PADA PROLIFERASI ADIPOSE MESENCHYMAL STEM CELL DENGAN SCAFFOLD NANOFIBER PHA/SILK DENGAN BERBAGAI KOMBINASI. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.
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Abstract
Introduction : Degenerative diseases, one of which is cartilage damage due to age, unhealthy lifestyles, genetics, obesity and smoking, are still common and it can cause bone cartilage to weaken. This research was conducted to determine adipose mesenchymal stem cells (ADSCs)’s ability with the addition of PHA/Silk nanofiber scaffolds to regenerate tissue which will then be used for regenerative therapy with minimal risks compared to operative therapy. Method : ADSCs originate from human adipose tissue cells taken using a liposuction procedure. Adipose cells will then be processed into ADSCs. Then ADSCs were cultured and passaged. ADSCs were combined with PHA (Polyhydroxyalkanoates)/Silk nanofiber scaffolds with ratios of 4:0, 3:1, 1:1, 1:3, and 0:4. ADSCs with scaffolds were subjected to RNA isolation, cDNA synthesis, and Real Time PCR was carried out to detect whether there was expression of the Bax gene (pro-apoptotic gene) in each treatment. Result : The fold change result of the control without scaffold is 1, ADSCs + PHA/Silk scaffold 4:0 and 1:1 is 0.001, ADSCs + PHA/Silk 3:1 scaffold is 0.002, ADSCs + PHA/Silk 1:3 scaffold is 0.948, and ADSCs + PHA/Silk 0:4 scaffold is 0.851. Conclusion : The results showed a decrease in Bax gene expression in all treatments. High expression of the Bax gene indicates a high level of apoptosis. Apoptosis can initiate cell proliferation (Apoptosis-Induced Proliferation) through activation of the JNK pathway which involves MAPK (Mitogen-Activated Kinases). ADSCs + PHA/Silk 1:3 scaffold had the highest level of Bax gene expression, indicating that their proliferation rate was also increased. ADSCs + PHA/Silk 4:0 and 1:1 scaffolds had the lowest levels of Bax gene expression, indicating that their proliferation levels were low.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Additional Information: | [No. Panggil: 2010211090] [Pembimbing 1: Andri Pramesyanti Pramono] [Pembimbing 2: Sri Wahyuningsih] [Penguji: Uswatun Hasanah] |
| Uncontrolled Keywords: | ADSCs, Bax, Apoptosis, PHA/Silk Scaffold |
| Subjects: | Q Science > QR Microbiology R Medicine > R Medicine (General) |
| Divisions: | Fakultas Kedokteran > Program Studi Kedokteran (S1) |
| Depositing User: | R. Regina Zaafira Desnita Purnawan |
| Date Deposited: | 22 Mar 2024 08:52 |
| Last Modified: | 22 Mar 2024 08:52 |
| URI: | http://repository.upnvj.ac.id/id/eprint/28541 |
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