ANALISIS GEN KOLAGEN TIPE II BIOMARKER KONDROGENESIS ADIPOSE MESENCHYMAL STEM CELL DENGAN PENAMBAHAN PLATELET RICH PLASMA PADA SCAFFOLD NANOFIBER PHA DAN SILK.

Andrea Salsabila Rosadi, . (2024) ANALISIS GEN KOLAGEN TIPE II BIOMARKER KONDROGENESIS ADIPOSE MESENCHYMAL STEM CELL DENGAN PENAMBAHAN PLATELET RICH PLASMA PADA SCAFFOLD NANOFIBER PHA DAN SILK. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

Cartilage is avascular tissue, making it difficult to repair itself when damaged. Tissue engineering can be use for repair cartilage damage. This research aims to explore the potential of adipose-derived stem cells (ADSCs), a combination of nanofiber polyhydroxyalkanoate (PHA) and silk scaffold, with the addition of platelet-rich plasma (PRP) as components of tissue engineering. ADSCs were cultured on a silk and PHA/silk nanofiber scaffold with a 3:1 ratio in Dulbecco's Modified Eagle's Medium (DMEM), both with and without the addition of 10% platelet-rich plasma (PRP) for 21 days to assess their potential to differentiate into cartilage, as indicated by the expression of type II collagen. Gene expression was analyzed using RT-qPCR. The highest increase in type II collagen gene expression occurred in the ADSCs cultured on the silk scaffold without 10% PRP addition, showing a 6.04-fold upregulation. In contrast, the treatment group with the addition of 10% PRP exhibited a 2.59-fold increase compared to the control. The ADSCs cultured on the mixed nanofiber scaffold of PHA and silk with a 3:1 ratio experienced a decrease in type II collagen gene expression, both with and without 10% PRP supplementation. This study indicates that 10% PRP addition to the chondrogenic culture media of ADSCs on a PHA and silk nanofiber scaffold can enhance the expression of type II collagen genes, although not to its maximum potential. This result could be attributed to the diverse preparation methods of PRP.

Item Type:	Thesis (Skripsi)
Additional Information:	[No. Panggil: 2010211018] [Pembimbing 1: Andri Pramesyanti Pramono] [Pembimbing 2: Abdul Kolib] [Penguji: Meiskha Bahar]
Uncontrolled Keywords:	Adipose Mesenchymal Stem Cell, Chondrogenesis, PHA, PRP, Scaffold nanofiber, Silk Fibroin, Type II Collagen
Subjects:	R Medicine > R Medicine (General)
Divisions:	Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User:	Andrea Salsabila Rosadi
Date Deposited:	14 Mar 2024 07:17
Last Modified:	14 Mar 2024 07:17
URI:	http://repository.upnvj.ac.id/id/eprint/28413

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