ANALISIS EKSPRESI GEN BCL-2 PADA KULTUR SEL PUNCA ADIPOSA MESENKIMAL DENGAN PENAMBAHAN MADU (Tetragonula sp.) DAN ROYAL JELLY (Apis mellifera)

Melati Nurulita Alvina, . (2023) ANALISIS EKSPRESI GEN BCL-2 PADA KULTUR SEL PUNCA ADIPOSA MESENKIMAL DENGAN PENAMBAHAN MADU (Tetragonula sp.) DAN ROYAL JELLY (Apis mellifera). Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

Adipose mesenchymal stem cells are used in various clinical trials of patient treatment. To provide treatment to patients, these cells must first be cultured on Dulbecco’s Modified Eagle Medium (DMEM) with FBS supplements. Fetal Bovine Serum (FBS) has a disadvantage, it can be contaminated by prion proteins, endotoxins, various types of microbes, immunoglobulins, and viruses. Regarding their composition, honey and royal jelly have the potential to be an alternative to FBS. According to previous studies, Tetragonula sp. honey and Apis mellifera royal jelly may induce cell proliferation, but the event of apoptosis has not been confirmed. Bcl-2 is a marker of apoptosis, as an anti-apoptosis. This study aims to analyze the effect of Tetragonula sp. honey and Apis mellifera royal jelly addition in DMEM against adipose mesenchymal stem cell apoptosis through Bcl-2 gene expression. This study used true experimental methods. Samples were obtained from the liposuction procedure of healthy adult patients cultured with honey and royal jelly 0.05% and 0.1% concentrations, as well as FBS 10%, then real-time PCR tests were carried out. The addition of honey and royal jelly resulted in an optimum percentage of cell proliferation at 0.1% concentration and did not change the normal (spindle-shaped) morphology of adipose mesenchymal stem cells. The highest expression of the Bcl-2 gene was obtained at 0.05% concentration, but at 0.1% concentration, the expression was lower than the controls. It can be concluded that the addition of honey and royal jelly to DMEM and FBS influences the expression of the Bcl-2 gene. This study is expected to represent the incidence of apoptosis that follows the process of cell proliferation in the addition of honey and royal jelly.

Item Type: Thesis (Skripsi)
Additional Information: [No.Panggil: 1910211080] [Pembimbing 1: Andri Pramesyanti Pramono] [Pembimbing 2: Hikmah Muktamiroh] [Penguji: Mila Citrawati]
Uncontrolled Keywords: Bcl-2, DMEM, FBS, Honey, Royal Jelly, Adipose Mesenchymal Stem Cells
Subjects: Q Science > QR Microbiology
R Medicine > R Medicine (General)
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: Melati Nurulita Alvina
Date Deposited: 23 Aug 2023 07:04
Last Modified: 23 Aug 2023 07:04
URI: http://repository.upnvj.ac.id/id/eprint/24050

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