UJI PROLIFERASI MTS ASSAY TERHADAP ADIPOSE MESENCHYMAL STEM CELLS PADA MEDIA KULTUR DMEM BEBAS SERUM DENGAN PENAMBAHAN MADU Tetragonula sp DAN ROYAL JELLY Apis mellifera

Muhammad Dhaffa, . (2022) UJI PROLIFERASI MTS ASSAY TERHADAP ADIPOSE MESENCHYMAL STEM CELLS PADA MEDIA KULTUR DMEM BEBAS SERUM DENGAN PENAMBAHAN MADU Tetragonula sp DAN ROYAL JELLY Apis mellifera. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

[img] Text
ABSTRAK.pdf
Download (13kB)
[img] Text
AWAL.pdf
Download (487kB)
[img] Text
BAB 1.pdf
Download (95kB)
[img] Text
BAB 2.pdf
Restricted to Repository UPNVJ Only
Download (250kB)
[img] Text
BAB 3.pdf
Restricted to Repository UPNVJ Only
Download (161kB)
[img] Text
BAB 4.pdf
Restricted to Repository UPNVJ Only
Download (327kB)
[img] Text
BAB 5.pdf
Download (79kB)
[img] Text
DAFTAR PUSTAKA.pdf
Download (157kB)
[img] Text
RIWAYAT HIDUP.pdf
Restricted to Repository UPNVJ Only
Download (24kB)
[img] Text
LAMPIRAN.pdf
Restricted to Repository UPNVJ Only
Download (1MB)
[img] Text
HASIL PLAGIARISME.pdf
Restricted to Repository staff only
Download (110kB)
[img] Text
ARTIKEL KI.pdf
Restricted to Repository staff only
Download (712kB)

Abstract

Adipose mesenchymal stem cells (AMSCs) are multipotent stem cells with potency for tissue therapy. Isolated primary mesenchymal stem cells are low in numbers, so in vitro expansion in culture medium is needed. DMEM and fetal bovine serum (FBS) are required for the medium. When used for AMSCs culture supplement, FBS has its disadvantages, one of them is the expression of Neu5Gc xenoantigen, which is harmful for human. Honey and royal jelly are known to have the potential composition needed as an alternative of FBS. This study aimed to determine the effect of serum-free DMEM with addition of Tetragonula sp honey and Apis mellifera royal jelly toward AMSCs proliferation. The research design used true experimental methods. Sample taken from a non-comorbid, healthy adult patient that underwent liposuction. AMSCs cultured with various concentrations of honey and royal jelly (0,05% and 0,1%), and the results of proliferation rates are determined with 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay or MTS assay. AMSCs cultured in 0,05% honey and royal jelly with additional 10% FBS has higher proliferation percentage than concentration groups of 0,1% with additional 10% FBS (p<0.05), 0,05% (p<0.05), 0,1% (p<0.05) and negative control (p<0.05). Nevertheless, the proliferation percentage still lower than positive control, which contain DMEM and 10% FBS without honey and royal jelly. Glucose composition in honey and royal jelly can inhibit MSCs proliferation, because of the formation of reactive oxygen species (ROS) induced by glucose excess. Further study about separate effects of honey and royal jelly usage as DMEM supplement and a safe amount of glucose threshold for AMSCs proliferation are needed, to make an optimal FBS alternative.

Item Type: Thesis (Skripsi)
Additional Information: [No.Panggil: 1810211056] [Pembimbing 1: Andri Pramesyanti Pramono] [Pembimbing 2: Niniek Hardini] [Penguji: Yudhi Nugraha]
Uncontrolled Keywords: Honey, Royal Jelly, DMEM, FBS, Adipose Mesenchymal Stem Cells
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: Muhammad Dhaffa
Date Deposited: 13 Sep 2022 02:51
Last Modified: 13 Sep 2022 02:51
URI: http://repository.upnvj.ac.id/id/eprint/20000

Actions (login required)

View Item View Item