POTENSI MADU TETRAGONULA SP. SEBAGAI CRYOPRESERVATIVE ADDITIVE UNTUK PENYIMPANAN SEL MESENKIMAL ADIPOSA DENGAN CRYOPROTECTIVE

Ario Seto, . (2022) POTENSI MADU TETRAGONULA SP. SEBAGAI CRYOPRESERVATIVE ADDITIVE UNTUK PENYIMPANAN SEL MESENKIMAL ADIPOSA DENGAN CRYOPROTECTIVE. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

The use of Dimethyl Sulfoxide (DMSO) can be applied in stem cell engineering with a cryopreservation process. To achieve optimal viability after cryopreservation, DMSO was substituted with Tetragonula sp. This study aimed to determine the effect of adding Tetragonula sp honey as a cryopreservative additive on the viability of mesenchymal adipose stem cells (ADSC). The research design used a pure experimental method. Samples were obtained from healthy people. ADSC cells were cryopreserved with various concentrations of 0.5% and 0.25% honey for 1 day then cell viability was measured using the MTS assay. The media with the addition of Tetragonula sp 0.25% honey (p-value = 0.318; ) did not exceed the viability of the standardized cryoprotectant with DMSO, but the viability of the cryoprotectant media with the addition of 0.5% Tetragonula sp honey had a significant difference compared to the viability of the medium. DMSO (p = 0.038). Sugar as the main composition in honey cryoprotectant media can reduce cell viability after cryopreservation. Effective and safe alternatives to DMSO for cryopreservation should be developed..

Item Type: Thesis (Skripsi)
Additional Information: [No Panggil : 1810211094] [Pembimbing 1 : Andri Pramesyanti Pramono] [Pembimbing 2 : Ryan Herardi] [Penguji : Karina]
Uncontrolled Keywords: DMSO; ADSC; Tetragonula sp
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: Ario Seto
Date Deposited: 13 Sep 2022 04:11
Last Modified: 13 Sep 2022 04:11
URI: http://repository.upnvj.ac.id/id/eprint/19875

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