PENGARUH PEMBERIAN EKSTRAK DAUN UNGU (Graptophyllum pictum (L.) Griff) TERHADAP REGENERASI SEL GLIA PADA TIKUS MODEL STROKE DENGAN METODE IMUNOHISTOKIMIA

Karuniawan Yusuf Noviandi, . (2026) PENGARUH PEMBERIAN EKSTRAK DAUN UNGU (Graptophyllum pictum (L.) Griff) TERHADAP REGENERASI SEL GLIA PADA TIKUS MODEL STROKE DENGAN METODE IMUNOHISTOKIMIA. Tesis thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

Background: Ischemic stroke is a leading cause of death in Indonesia and causes brain tissue damage through oxidative stress, inflammation, and neuronal cell death. One biomarker of brain damage is Glial Fibrillary Acidic Protein (GFAP), which increases in response to astrogliosis. Graptophyllum pictum (L.) Griff is an herbal plant containing flavonoids, tannins, and saponins, which act as antioxidants, potentially reducing oxidative stress and supporting glial cell regeneration after ischemic stroke. Objective: To analyze the effect of purple leaf extract on glial cell regeneration and neurophil spongiosis in the brain tissue of Rattus norvegicus Sprague Dawley rats induced by ischemic stroke. Methods: This study used a true experimental design with a post-test only control group. Male Sprague Dawley rats were induced with stroke using the Middle Cerebral Artery Occlusion (MCAO) method. The treatment group was given several doses of purple leaf extract, while the control group received no therapy. After the treatment period, brains were removed for hematoxylin-eosin histology to assess neurophil spongiosis and GFAP immunohistochemistry to assess reactive astrocyte expression as an indicator of glial cell regeneration. Results: The group of mice given purple leaf extract showed a decrease in the degree of neurophil spongiosis and decreased GFAP expression compared to the control group, indicating a reduced inflammatory process and increased glial cell regeneration after ischemic stroke. The therapeutic effect increased with increasing extract dose. Conclusion: Purple leaf extract (Graptophyllum pictum) is effective in enhancing glial cell regeneration and reducing brain tissue damage in a rat model of ischemic stroke through an antioxidant mechanism. Purple leaf has the potential to be used as an adjunct herbal-based therapy for stroke treatment.

Item Type: Thesis (Tesis)
Additional Information: [No. Panggil: 2320223002] [Pembimbing 1: Feda Anisah Makkiyah] [Pembimbing 2: Taufiq Fredrik Pasiak] [Penguji 1: Maria Selvester Thadeus] [Penguji 2: Muttia Amalia]
Uncontrolled Keywords: Ischemic Stroke, Astrogliosis, GFAP, Neutrophilic Spongiosis, Purple Leaf Extract (Graptophyllum Pictum)
Subjects: R Medicine > R Medicine (General)
R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: KARUNIAWAN YUSUF NOVIANDI
Date Deposited: 28 Apr 2026 07:54
Last Modified: 28 Apr 2026 07:54
URI: http://repository.upnvj.ac.id/id/eprint/42728

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