UJI EFEKTIVITAS EKSTRAK DAUN TEH HIJAU (Camellia sinensis) DENGAN METODE ULTRASONIC ASSISTED EXTRACTION (UAE) TERHADAP PENGHAMBATAN PERTUMBUHAN JAMUR Malassezia furfur SECARA IN VITRO

Mahera Alfiani Rachmania, . (2025) UJI EFEKTIVITAS EKSTRAK DAUN TEH HIJAU (Camellia sinensis) DENGAN METODE ULTRASONIC ASSISTED EXTRACTION (UAE) TERHADAP PENGHAMBATAN PERTUMBUHAN JAMUR Malassezia furfur SECARA IN VITRO. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

Malassezia furfur, the cause of Pityriasis versicolor, poses resistance challenges in Indonesia. Green tea leaf (Camellia sinensis) extract offers a safer antifungal alternative containing tannins, flavonoids, alkaloids, and triterpenoids. This experimental study evaluated C. sinensis extract effectiveness against M. furfur in vitro using a post-test only control group design. Extracts were prepared via Ultrasonic Assisted Extraction (UAE) method at 10%, 15%, 20%, and 25%, with sterile aquadest and 2% as controls. Antifungal activity was assessed using the agar well diffusion method on Saburoud Dextrose Agar (SDA) by measuring inhibition zone after 24 and 48 hours of incubation. Inhibition was generally moderate at 24 hours but weakened at 48 hours, except at 20% and 25% concentrations. Factors such as variations in extract concentration, the amount of active secondary metabolite compounds, and the incubation periode resulted in differences in effectiveness. A p-value < 0,05 was obtained in the Kruskal-Wallis analysis for the 24-hour data, indicating that the administration of green tea leaf extract at various concentrations produced significantly different inhibitory effects on M. furfur. Based on Post-Hoc analysis, it was found that the 20% concentration was the most effective concentration for inhibiting M.furfur.

Item Type: Thesis (Skripsi)
Additional Information: [No.Panggil: 2210211127] [Pembimbing 1: Yuni Setyaningsih] [Pembimbing 2: Adi Sukrisno] [Penguji: Fajriati Zulfa]
Uncontrolled Keywords: Antifungal; Green tea leaf extract; Malassezia furfur; Pityriasis versicolor; Ultrasonic Assisted Extraction
Subjects: R Medicine > R Medicine (General)
R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: MAHERA ALFIANI RACHMANIA
Date Deposited: 22 Apr 2026 02:26
Last Modified: 22 Apr 2026 02:26
URI: http://repository.upnvj.ac.id/id/eprint/41767

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