PERBANDINGAN EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN UNGU (Graptophyllum pictum (L.) Griff) DAN ISOLAT ACTINOMYCETES MENGGUNAKAN METODE ULTRASOUND-ASSISTED EXTRACTION TERHADAP PERTUMBUHAN Staphylococcus aureus

Angga Prawira, . (2024) PERBANDINGAN EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN UNGU (Graptophyllum pictum (L.) Griff) DAN ISOLAT ACTINOMYCETES MENGGUNAKAN METODE ULTRASOUND-ASSISTED EXTRACTION TERHADAP PERTUMBUHAN Staphylococcus aureus. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

[img] Text
ABSTRAK.pdf
Download (62kB)
[img] Text
AWAL.pdf
Download (632kB)
[img] Text
BAB 1.pdf
Restricted to Repository UPNVJ Only
Download (19kB)
[img] Text
BAB 2.pdf
Restricted to Repository UPNVJ Only
Download (480kB)
[img] Text
BAB 3.pdf
Restricted to Repository UPNVJ Only
Download (156kB)
[img] Text
BAB 4.pdf
Restricted to Repository UPNVJ Only
Download (197kB)
[img] Text
BAB 5.pdf
Download (10kB)
[img] Text
DAFTAR PUSTAKA.pdf
Download (197kB)
[img] Text
RIWAYAT HIDUP.pdf
Restricted to Repository UPNVJ Only
Download (30kB)
[img] Text
LAMPIRAN.pdf
Restricted to Repository UPNVJ Only
Download (2MB)
[img] Text
HASIL PLAGIARISME.pdf
Restricted to Repository staff only
Download (3MB)
[img] Text
ARTIKEL KI.pdf
Restricted to Repository staff only
Download (2MB)

Abstract

Graptophyllum pictum (L.) Griff and Actinomycetes are natural resources known in Indonesia as medicinal plants and antimicrobial agents. G. pictum extract contains bioactive compounds with antibacterial properties, while Actinomycetes produce secondary metabolites effective against bacteria. This study evaluates and compares the antimicrobial effectiveness of G. pictum extract and Actinomycetes isolates extracted and isolated using the Ultrasound-Assisted Extraction (UAE) method against Staphylococcus aureus. The method used is a laboratory experiment with a comparative approach, involving the extraction of G. pictum and isolation of Actinomycetes, followed by antimicrobial activity testing using the well diffusion method. Results showed that G. pictum extract inhibited the growth of S. aureus at concentrations of 40%, 50%, and 60% with average inhibition zone diameters of 7.475 mm; 9.45 mm; and 10.05 mm; respectively. In comparison, Actinomycetes isolates showed average inhibition zone diameters of 5.35 mm at 40% concentration, 6.85 mm at 50% concentration, and 4.325 mm at 60% concentration. The Mann-Whitney test showed significant differences at 40% (p = 0.043) and 60% concentrations (p = 0.021), but not at 50% concentration (p = 0.248). The conclusion is that G. pictum extract has higher potential as an antimicrobial agent compared to Actinomycetes isolates against S. aureus. The UAE technique used in this study increases the efficiency of extracting active compounds from G. pictum and isolating Actinomycetes bacteria, resulting in higher concentrations of active compounds and secondary metabolites from both sources. It is hoped that this research can contribute to the development of alternative treatments for bacterial infections using natural materials.

Item Type: Thesis (Skripsi)
Additional Information: [No.Panggil: 2110211031] [Pembimbing 1: Meiskha Bahar] [Pembimbing 2: Yuni Setyaningsih] [Penguji: Maria Selvester Thadeus]
Uncontrolled Keywords: Actinomycetes, Antimicrobial, Graptophyllum pictum (L.) Griff, Staphylococcus aureus, Ultrasound-Assisted Extraction
Subjects: Q Science > QR Microbiology
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: ANGGA PRAWIRA
Date Deposited: 17 Mar 2025 01:33
Last Modified: 17 Mar 2025 01:33
URI: http://repository.upnvj.ac.id/id/eprint/34401

Actions (login required)

View Item View Item