ANALISIS KADAR MARKA GEN PENUAAN TRANSFORMING GROWTH FACTOR-β (TGF-β) TERHADAP WAKTU PAPARAN ULTRAVIOLET B PADA RNA SEL FIBROBLAS

SYIFA WINDI ADELIA, . (2024) ANALISIS KADAR MARKA GEN PENUAAN TRANSFORMING GROWTH FACTOR-β (TGF-β) TERHADAP WAKTU PAPARAN ULTRAVIOLET B PADA RNA SEL FIBROBLAS. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

[img] Text
ABSTRAK.pdf
Download (274kB)
[img] Text
AWAL.pdf
Download (1MB)
[img] Text
BAB 1.pdf
Download (490kB)
[img] Text
BAB 2.pdf
Restricted to Repository UPNVJ Only
Download (1MB)
[img] Text
BAB 3.pdf
Restricted to Repository UPNVJ Only
Download (947kB)
[img] Text
BAB 4.pdf
Restricted to Repository UPNVJ Only
Download (1MB)
[img] Text
BAB 5.pdf
Download (473kB)
[img] Text
DAFTAR PUSTAKA.pdf
Download (424kB)
[img] Text
RIWAYAT HIDUP.pdf
Restricted to Repository UPNVJ Only
Download (197kB)
[img] Text
ARTIKEL.pdf
Restricted to Repository staff only
Download (407kB)
[img] Text
LAMPIRAN.pdf
Restricted to Repository UPNVJ Only
Download (511kB)
[img] Text
HASIL PLAGIARISME.pdf
Restricted to Repository staff only
Download (11MB)

Abstract

Indonesia is a tropical country where the intensity of sunlight is high and occurs throughout the year. Exposure to sunlight, especially ultraviolet rays, causes premature aging. Aging can be observed at the molecular level, where changes occur in genes, including TGF-β. TGF-β is a cytokine that regulates various cellular processes, such as embryonic development, tissue homeostasis, and the control of cell growth, proliferation, and apoptosis, as well as various pathological conditions such as immune dysfunction, inflammation, oncogenesis, and fibrosis. The aim of this study is to analyze the differences in TGF-β levels in fibroblast RNA before and after induction by ultraviolet B (UVB) rays. This research was conducted by measuring TGF-β levels using qRT-PCR on RNA from fibroblast cells in palatal tissue that were not exposed to UVB and those exposed for 24, 48, and 96 minutes at a dose of 144 mJ/cm² with a wavelength of 310 nm. The average TGF-β level before UVB exposure was found to be 1.000130, while after 24 minutes of exposure it was 0.00792, after 48 minutes it was 0.00475, and after 96 minutes it was 0.00186.There was a significant change and decrease in the average relative expression level of the TGF-β gene compared to GAPDH, as tested by the One-Way ANOVA method, where the p-value was < 0.05. This indicates that the effect of UVB exposure is likely greater in dermal because fibroblasts in palatal tissue have better healing capabilities

Item Type: Thesis (Skripsi)
Additional Information: [No.Panggil: 2110211081] [Pembimbing 1: Karina] [Pembimbing 2: Isniani Ramadhani] [Penguji : Muttia Amalia]
Uncontrolled Keywords: duration of UVB radiation, fibroblast, photoaging, transforming growth factor-β, ultraviolet B
Subjects: R Medicine > R Medicine (General)
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: SYIFA WINDI ADELIA
Date Deposited: 17 Jan 2025 07:41
Last Modified: 17 Jan 2025 07:41
URI: http://repository.upnvj.ac.id/id/eprint/34190

Actions (login required)

View Item View Item