Kanaya Nisa Salsabila, . (2025) ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI ISOLAT BAKTERI ENDOFIT BATANG KROKOT (Portulaca oleracea L.) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.
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Abstract
Objective Antimicrobial resistance has intensified the search for novel antibacterial agents, including those derived from plant-associated endophytic bacteria. This study aimed to isolate endophytic bacteria from the stem of Portulaca oleracea L. and evaluate their antibacterial activity against Staphylococcus aureus ATCC 6583 and Escherichia coli ATCC 25922. Method This in vitro experimental study included isolation, purification, morphological and biochemical characterization, Gram and endospore staining, and antibacterial testing using the Kirby–Bauer disc diffusion method. Inhibition zone diameters were statistically analyzed using the Kruskal–Wallis and Mann–Whitney U tests. Results Two endophytic bacterial isolates, K1-A and K5-A, were obtained and exhibited characteristics suggestive of Bacillus spp. Isolate K1-A exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli, whereas K5-A showed activity only against Escherichia coli. Statistical analysis indicated significant differences among treatment groups (p < 0.001), with K1-A demonstrating higher antibacterial activity than K5-A. Conclusion In conclusion, endophytic bacteria isolated from the stem of Portulaca oleracea L. exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli, with isolate K1-A showing higher activity than K5-A. These findings indicate the potential of endophytic bacteria from P. oleracea as a source of natural antibacterial compounds.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Additional Information: | [No.Panggil 2210211086] [Pembimbing 1: Seftiwan Pratami Djasfar] [Pembimbing 2: Hikmah Muktamiroh] [Penguji : Meiskha Bahar] |
| Uncontrolled Keywords: | Antibacterial Activity, Endophytic Bacteria, Escherichia coli, Portulaca oleracea, Staphylococcus aureus |
| Subjects: | Q Science > QR Microbiology R Medicine > R Medicine (General) |
| Divisions: | Fakultas Kedokteran > Program Studi Kedokteran (S1) |
| Depositing User: | KANAYA NISA SALSABILA |
| Date Deposited: | 13 Apr 2026 06:30 |
| Last Modified: | 13 Apr 2026 06:30 |
| URI: | http://repository.upnvj.ac.id/id/eprint/41947 |
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