Afkaar Muhammad, . (2026) EFEK PEMBERIAN SEL NATURAL KILLER (NK) DALAM INDUKSI APOPTOSIS PADA KANKER PAYUDARA MCF-7 SECARA IN VITRO. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.
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Abstract
Breast cancer remains one of the most common malignancies in women and is often treated with conventional therapies that still possess limitations and side effects. Therefore, administration of natural killer (NK) cells toward breast cancer cell lines MCF-7 in vitro serves as a preliminary modality for subsequent clinical application. Objective: This study aimed to investigate the effect of NK cell administration on MCF-7 breast cancer cells in vitro. The observed effects included cell viability, apoptosis rates, and response comparison between MCF-7 cells as effector targets with a predetermined effector-to-target cell ratio in vitro. This experimental research was conducted in HayandraLab, utilizing frozen NK cell stocks and cancer cell stocks preserved under proper laboratory conditions. Methods: This was a true experimental study with a descriptive and quantitative approach. NK cells and MCF-7 cells were co-cultured and divided into four experimental groups based on the effector-to-target cell ratio. The total cell concentration used was determined prior to treatment. Flow cytometry analysis was performed to assess viability and apoptosis of MCF-7 cells. NK cell characterization and target recognition were evaluated using flow cytometry, while qRT-PCR was used to measure the expression of BCL-2 and p53 genes. Data from viability and apoptosis assays were processed using descriptive analysis, whereas qRT-PCR data were analyzed with One-way ANOVA. Results: The results demonstrated decreased viability and increased apoptosis in MCF-7 cells following co-culture with NK cells across different effector-to-target ratios. The highest apoptotic effect was observed in treatment group K1 (E:T Ratio 10:1). qRT-PCR data showed significant changes in gene expression of BCL-2 and p53, indicating statistically significant differences between treatment groups and controls. Increased BCL-2 expression (p < 0.01) was observed in treatment group K2 (20:1), while p53 expression showed a significant increase (p < 0.001) in treatment group K3 (50:1). Conclusion: It can be concluded that NK cell administration induces significant apoptotic responses and alters gene expression in MCF-7 breast cancer cells in vitro. NK cell effects may involve both direct cytotoxic mechanisms as well as apoptosis signaling pathways associated with BCL-2 and p53 regulation.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Additional Information: | [No.Panggil: 2210211122] [Pembimbing 1: Karina] [Pembimbing 2: Ria Maria Theresa] [Penguji: Maulidya Ayudika Dandanah] |
| Uncontrolled Keywords: | Apoptosis, in vitro, MCF-7, natural killer cells, viability |
| Subjects: | R Medicine > RD Surgery R Medicine > RZ Other systems of medicine |
| Divisions: | Fakultas Kedokteran > Program Studi Kedokteran (S1) |
| Depositing User: | AFKAAR MUHAMMAD |
| Date Deposited: | 16 Apr 2026 10:19 |
| Last Modified: | 24 Apr 2026 02:00 |
| URI: | http://repository.upnvj.ac.id/id/eprint/41863 |
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