Shelly Asna Fawadhila, . (2026) ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI ISOLAT BAKTERI ENDOFIT AKAR TANAMAN PATIKAN KEBO (EUPHORBIA HIRTA L.) TERHADAP ESCHERICHIA COLI DAN STAPHYLOCOCCUS AUREUS. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.
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Abstract
Objective Euphorbia hirta L. is known as a medicinal plant with potential antibacterial properties, one of which is associated with the presence of endophytic bacteria living within plant tissues. However, the utilization of endophytic bacteria from plants as antibacterial agents remains limited. Therefore, this study aims to determine the characteristics and antibacterial activity of endophytic bacterial isolates isolated from the roots of patikan kebo (Euphorbia hirta L.) against the test bacteria Escherichia coli and Staphylococcus aureus. Method This research was conducted using an experimental laboratory design. Isolation of endophytic bacteria was carried out from root samples of Euphorbia hirta L. that had undergone surface sterilization. The obtained isolates were phenotypically characterized including colony morphology, Gram staining, catalase test, endospore staining, and biochemical tests. Preliminary screening of antibacterial activity was performed using the cross streak method, followed by further testing of selected isolates using the disc diffusion method against the test bacteria Escherichia coli and Staphylococcus aureus. Results A total of 26 endophytic bacterial isolates were successfully isolated from the roots of Euphorbia hirta L. Preliminary screening results showed that eight isolates had antibacterial potential. Gram staining of the potential isolates showed a predominance of Gram-positive bacteria, with seven isolates (87.5%) classified as Gram-positive and one isolate (12.5%) as Gram-negative. Phenotypic characterization showed that seven isolates had similarities to the genus Bacillus and one isolate showed characteristics resembling Pseudomonas. Antibacterial activity varied among isolates, with the highest inhibition against Staphylococcus aureus of 19.35 mm and against Escherichia coli of 5.32 mm. Conclusion Endophytic bacteria isolated from the roots of patikan kebo (Euphorbia hirta L.) have phenotypic diversity and show potential antibacterial activity, particularly against Staphylococcus aureus, so that they have the potential to be developed as a source of natural antibacterial agents.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Additional Information: | [No.Panggil : 2210211023] [Pembimbing 1: Seftiwan Pratami Djasfar] [Pembimbing 2: Agneta Irmarahayu] [Penguji : Meiskha Bahar] |
| Uncontrolled Keywords: | antibacterial; Bacillus; endophytic bacteria; Escherichia coli; Euphorbia hirta L.; phenotypic; Staphylococcus aureus; medicinal plant |
| Subjects: | Q Science > QR Microbiology R Medicine > R Medicine (General) R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Kedokteran > Program Studi Kedokteran (S1) |
| Depositing User: | SHELLY ASNA FAWADHILA |
| Date Deposited: | 31 Mar 2026 03:57 |
| Last Modified: | 31 Mar 2026 03:57 |
| URI: | http://repository.upnvj.ac.id/id/eprint/41801 |
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