ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI ISOLAT BAKTERI ENDOFIT BATANG TANAMAN PATIKAN KEBO (Euphorbia hirta L.) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Escherichia coli

Syafa Indesqia Putri Indiawan, . (2025) ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI ISOLAT BAKTERI ENDOFIT BATANG TANAMAN PATIKAN KEBO (Euphorbia hirta L.) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Escherichia coli. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

Background Antimicrobial resistance has become a major global health threat, prompting the search for new antibacterial sources, including plant-derived endophytic bacteria. Euphorbia hirta L. is known to contain antimicrobial secondary metabolites; however, data on the antibacterial activity of endophytic bacteria isolated from its stem remain limited. This study aimed to isolate and identify endophytic bacteria from the stem of E. hirta and to evaluate their antibacterial activity against Staphylococcus aureus and Escherichia coli. Method A true experimental study was conducted by isolating endophytic bacteria from E. hirta stems using the direct culture method on Nutrient Agar, followed by macroscopic and microscopic characterization, biochemical tests, and endospore staining. Identification of bacterial genera was performed using Bergey’s Manual of Determinative Bacteriology. Antibacterial activity was assessed using the disc diffusion method to measure inhibition zones in comparison with positive control (chloramphenicol 30 μg) and negative control (distilled water). Result Five endophytic bacterial isolates were obtained (S1, S2, S3, S4, S5). However, S2 and S3 exhibited characteristics similar to S4, resulting in the selection of three isolates (S1, S4, S5) for further analysis. All three isolates showed characteristics consistent with the genus Bacillus sp. Against S. aureus, the largest inhibition zone was produced by isolate S1 (4.75 mm), followed by S5 (3.25 mm) and S4 (0.75 mm), all categorized as having weak antibacterial activity. Against E. coli, the largest inhibition zone was produced by S4 (5.41 mm), followed by S1 (5.19 mm) and S5 (3.42 mm), categorized as moderate activity for S1–S4 and weak activity for S5. Conclusion The three endophytic bacterial isolates from the stem of Euphorbia hirta showed similarity to the genus Bacillus sp. and demonstrated antibacterial activity against S. aureus and E. coli.

Item Type: Thesis (Skripsi)
Additional Information: [No.Panggil: 2210211093] [Pembimbing: Meiskha Bahar] [Penguji 1: Adi Sukrisno] [Penguji 2: Seftiwan Pratami Djasfar]
Uncontrolled Keywords: antibacterial activity, endophytic bacteria, Euphorbia hirta L., Escherichia coli, Staphylococcus aureus
Subjects: Q Science > QR Microbiology
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: SYAFA INDESQIA PUTRI INDIAWAN
Date Deposited: 21 Apr 2026 07:34
Last Modified: 21 Apr 2026 07:34
URI: http://repository.upnvj.ac.id/id/eprint/41149

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