Halimatus Syadiyah, . (2025) BRINE SHRIMP LETHALITY TEST (BSLT) DARI EKSTRAK ETANOL 70% DAN ETANOL 96% DAUN UNGU (Graptophyllum pictum L.). Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.
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Abstract
The use of herbal medicine as an alternative treatment continues to increase, including purple leaves (Graptophyllum pictum L.) which have various biological effects. Active plant compounds are potentially toxic in high doses, so toxicity tests are needed to ensure their safety. Extraction of secondary metabolites is influenced by solvent polarity. This study analyzed the acute toxicity of 70% and 96% ethanol extracts of purple leaves against Artemia franciscana larvae using the Brine Shrimp Lethality Test (BSLT) method which is indicated by the LC50 value. The extract was obtained by maceration, followed by phytochemical screening, Total Flavonoid Content (TFC) and BSLT toxicity test using extract solution concentrations of 100, 300, 500, and 700 ppm. Both extracts positively contained active compounds of alkaloids, flavonoids, tannins, saponins, and triterpenoids. The TFC of 70% and 96% ethanol extracts were 25.9429 mgQE/g extract and 38.5714 mgQE/g extract, respectively. The LC50 value of 70% ethanol extract was 423.338 ppm and 96% ethanol was 313.079 ppm. Both extracts are included in the toxic (Meyer) and moderately toxic (Clarkson) categories. The Independent T-Test produced a value (Sig <0.05) which means there is a significant difference in the TFC and LC50 values between the two types of extracts.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Additional Information: | [No Panggil: 2110212039] [Pembimbing: Dhigna Luthfiyani Citra Pradana] [Penguji 1: Eldiza Puji Rahmi] [Penguji 2: Rika Revina] |
| Uncontrolled Keywords: | Artemia franciscana, BSLT, LC50, Purple Leaves, Toxicity Test |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Fakultas Kedokteran > Program Studi Farmasi (S1) |
| Depositing User: | HALIMATUS SYADIYAH |
| Date Deposited: | 06 Aug 2025 01:18 |
| Last Modified: | 06 Aug 2025 01:18 |
| URI: | http://repository.upnvj.ac.id/id/eprint/38299 |
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