ANALISIS GEN BIOMARKER ALKALINE PHOSPHATASE (ALP) PADA DIFERENESIASI OSTEOGENESIS ADIPOSE-DERIVED STEM CELLS DENGAN SCAFFOLD PHA DAN SILK

Vhokiya Maulidya Putri Angkotasan, . (2024) ANALISIS GEN BIOMARKER ALKALINE PHOSPHATASE (ALP) PADA DIFERENESIASI OSTEOGENESIS ADIPOSE-DERIVED STEM CELLS DENGAN SCAFFOLD PHA DAN SILK. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

ADSCs are good candidates for clinical applications to regenerate damaged tissues. ADSCs have never been tested using PHA and silk scaffolds towards osteogenesis differentiation. The general objective of this study was to analyze the expression of ALP gene indicating osteogenic differentiation of ADSCs in PHA and silk scaffold media with a ratio of 3:1 and 0:4. ADSCs were taken from adipose tissue through liposuction procedure at Hayandra clinic, then ADSCs were cultured and passivated and planted on PHA and Silk nanofiber scaffolds with a ratio of 3:1 and 0:4. Then observed for 21 days, and performed RT-PCR to see the expression of osteogenesis genes in the form of ALP. The results of Fold Change ADSCs + Stempro Osteogenesis differentiation kit is 1, ADSCs without scaffold is 0.37, ADSCs + scaffold PHA and Silk 0: 4 is 0.38, ADSCs + scaffold PHA and Silk 3: 1 is 1. Based on the research, the decrease in ALP gene expression in the scaffold PHA and Silk ratio 0: 4 is not better than the use of silk with low levels, too high silk levels have disadvantages in improving the mechanical properties of osteogenic features. ADSCs with PHA and Silk 1:3 scaffold have the highest level of ALP gene expression because PHA has a better structure and biocompatibility in supporting the osteogenesis process.

Item Type: Thesis (Skripsi)
Additional Information: [No.Panggil: 2010211009] [Pembimbing 1: Andri Pramesyanti Pramono] [Pembimbing 2: Abdul Kolib] [Penguji 1: Karina]
Uncontrolled Keywords: ADSCs, ALP, DMEM, FBS, Osteogenesis, PHA, Scaffold, Silk
Subjects: R Medicine > R Medicine (General)
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: Vhokiya Maulidya Putri Angkotasan
Date Deposited: 05 Mar 2024 04:21
Last Modified: 05 Mar 2024 04:21
URI: http://repository.upnvj.ac.id/id/eprint/28509

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