ANALISIS GEN CYTOCHROME OXYDASE I (COI), MOTILITAS, DAN VIABILITAS SPERMA TIKUS PUTIH (Rattus norvegicus) HIPERGLIKEMIA SETELAH PEMBERIAN EKSTRAK DAUN KELOR (Moringa oleifera)

Wafa Nabilah Nawawi, . (2024) ANALISIS GEN CYTOCHROME OXYDASE I (COI), MOTILITAS, DAN VIABILITAS SPERMA TIKUS PUTIH (Rattus norvegicus) HIPERGLIKEMIA SETELAH PEMBERIAN EKSTRAK DAUN KELOR (Moringa oleifera). Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

To investigate the effects of moringa leaf extract on white rat (Rattus norvegicus) sperm, the Cytochrome Oxidase I (COI) gene expression, motility, and viability were analyzed. This study utilized true experimental design as a type of experimental research. The post-test control group design was employed, where observations were made after the treatment. The research aimed to analyze the COI gene expression, motility, and viability of hyperglycemic rat sperm treated with Moringa oleifera (moringa leaf extract). The treatment group was then compared with the control group. The results of the research were observed through the relative quantification of gene expression using comparative threshold cycle (Ct) through RT-PCR. In group K3, the motility of motile sperm reached 52% and the largest percentage of live sperm viability, which is 77%, was also found in this group. Moreover, gene expression analysis showed values close to 1 (fold change) in group K2 when compared with the normal or K(-) treatment group. After administering moringa leaf extract, a significant correlation was found between COI gene expression, motility, and viability of white rat sperm (Rattus norvegicus).

Item Type:	Thesis (Skripsi)
Additional Information:	[No. Panggil: 2010211149] [Pembimbing 1: Uswatun Hasanah] [Pembimbing 2: Abdul Kolib] [Penguji: Cut Fauziah]
Uncontrolled Keywords:	COI, Moringa oleifera, Rattus norvegicus, comparative threshold cycle
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics
Divisions:	Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User:	Wafa Nabilah Nawawi
Date Deposited:	08 Mar 2024 08:42
Last Modified:	08 Mar 2024 08:42
URI:	http://repository.upnvj.ac.id/id/eprint/28396

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