ANALISIS GEN BIOMARKER KONDROGENESIS KOLAGEN TIPE II ADIPOSE MESENCHYMAL STEM CELL PADA SCAFFOLD NANOFIBER PHA/SILK DENGAN BERBAGAI RASIO

Hanan, . (2024) ANALISIS GEN BIOMARKER KONDROGENESIS KOLAGEN TIPE II ADIPOSE MESENCHYMAL STEM CELL PADA SCAFFOLD NANOFIBER PHA/SILK DENGAN BERBAGAI RASIO. Skripsi thesis, Universitas Pembangunan Nasional Veteran Jakarta.

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Abstract

Objective Damage to cartilage due to obesity, age, genetics, or injury leads to thinning and permanent damage of the cartilage. This research aims to determine the potential of adipose mesenchymal stem cells (ADSCs) in tissue regeneration, particularly in the chondrogenic pathway before their use in therapy for cartilage-deficient conditions to minimize operative risk. Method ADSCs were extracted from patient adipose tissue through a liposuction process. Lipoaspirate was processed, stem cells were isolated, and then cultured for 21 days. A combination of nanofiber biomaterial scaffold from Polyhydroxyalkanoates (PHA)/silk with a PHA/Silk ratio of 3:1 and 0:4 was used. After 21 days, chondrogenic potential was evaluated by analyzing the gene expression of chondrogenesis biomarkers, type II collagen, using real-time PCR. Result The fold change result for the positive control was 1. ADSCs + scaffold nanofiber PHA/Silk 3:1 showed a fold change of 0.01, ADSCs + scaffold nanofiber PHA/Silk 0:4 exhibited a fold change of 7.57, and the fold change for the negative control was 0.91. Conclusion Decrease in gene expression in the negative control was due to the lack of specific signals from the DMEM + FBS medium, which was not designed for chondrogenic differentiation. The reduced gene expression in ADSCs + scaffold PHA/Silk 3:1 was presumed to result from the hydrophobic nature of PHA P(3HB-co-3HHx), reducing serum protein absorption, cell adhesion, and inhibiting cell proliferation. In ADSCs + scaffold nanofiber PHA/Silk 0:4, the increased gene expression occurred because silk produced non-toxic degradation products, cells were cultured in a medium specifically designed for the chondrogenic pathway, and the significant role of the hydrophilic scaffold silk facilitated cell adhesion, electrostatic interaction ability, surface smoothness, and numerous hydrogen bonds. Keywords: ADSCs, Chondrogenesis, Collagen Type II, PHA/Silk Scaffold

Item Type: Thesis (Skripsi)
Additional Information: [No.Panggil: 2010211008] [Pembimbing 1: Andri Pramesyanti Pramono] [Pembimbing 2: Uswatun Hasanah] [Penguji: Cut Fauziah]
Uncontrolled Keywords: ADSCs, Chondrogenesis, Collagen Type II, PHA/Silk Scaffold
Subjects: R Medicine > R Medicine (General)
Divisions: Fakultas Kedokteran > Program Studi Kedokteran (S1)
Depositing User: Hanan -
Date Deposited: 07 Mar 2024 07:46
Last Modified: 07 Mar 2024 07:46
URI: http://repository.upnvj.ac.id/id/eprint/27878

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